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rabbit primary polyclonal antibody against cx43  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology rabbit primary polyclonal antibody against cx43
    Culture of packaging cells 293FT and production of lentivirus. (A) Morphology of 293FT cells before viral packaging. The packaging cells were shown in good condition; (B) Morphology of 293FT cells during ingathering. The pLenti-based expression vector containing gene of rat Cx43 or <t>MyoD</t> and the ViraPower packing were transfected into 293FT cell line to produce lentivirus. The viruses were ingathered after culturing for 72h.
    Rabbit Primary Polyclonal Antibody Against Cx43, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit primary polyclonal antibody against cx43/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    rabbit primary polyclonal antibody against cx43 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro"

    Article Title: Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro

    Journal: International Journal of Biomedical Science : IJBS

    doi:

    Culture of packaging cells 293FT and production of lentivirus. (A) Morphology of 293FT cells before viral packaging. The packaging cells were shown in good condition; (B) Morphology of 293FT cells during ingathering. The pLenti-based expression vector containing gene of rat Cx43 or MyoD and the ViraPower packing were transfected into 293FT cell line to produce lentivirus. The viruses were ingathered after culturing for 72h.
    Figure Legend Snippet: Culture of packaging cells 293FT and production of lentivirus. (A) Morphology of 293FT cells before viral packaging. The packaging cells were shown in good condition; (B) Morphology of 293FT cells during ingathering. The pLenti-based expression vector containing gene of rat Cx43 or MyoD and the ViraPower packing were transfected into 293FT cell line to produce lentivirus. The viruses were ingathered after culturing for 72h.

    Techniques Used: Expressing, Plasmid Preparation, Transfection

    Expression of rat Cx43 and MyoD after lentivirus transfection. MyoD and Cx43 expression levels in four groups of fibroblasts: non-treated fibroblast (N), MyoD gene-transfected fibroblast (M), Cx43 gene-transfected fibroblast (C) and MyoD-Cx43-transfected fibroblast (MC) were analyzed. (A) MyoD mRNA expression was determined by RT-PCR. The 254bp band is MyoD gene amplifying product. The figure shows that MyoD mRNA was highly expressed in the MyoD- and MyoD plus Cx43- transfected fibroblasts; (B) Cx43 mRNA expression was determined by RT-PCR. The 580 bp band indicates Cx43 gene PCR product. Panel B reveals that Cx43 mRNA was only detected highly in the Cx43- transfected fibroblasts. In addition, low level expression of Cx43 was found in MyoD gene-transfected group. Non-treated fibroblasts did not express mRNA of these two genes; (C) MyoD and (D) Cx43 protein level was analyzed by western blotting. GAPDH was used as a sample loading control. MyoD or Cx43 protein was expressed in the MyoD- or Cx43-transfected fibroblasts. Note low level expression of Cx43 was found in MyoD gene-transfected fibroblasts.
    Figure Legend Snippet: Expression of rat Cx43 and MyoD after lentivirus transfection. MyoD and Cx43 expression levels in four groups of fibroblasts: non-treated fibroblast (N), MyoD gene-transfected fibroblast (M), Cx43 gene-transfected fibroblast (C) and MyoD-Cx43-transfected fibroblast (MC) were analyzed. (A) MyoD mRNA expression was determined by RT-PCR. The 254bp band is MyoD gene amplifying product. The figure shows that MyoD mRNA was highly expressed in the MyoD- and MyoD plus Cx43- transfected fibroblasts; (B) Cx43 mRNA expression was determined by RT-PCR. The 580 bp band indicates Cx43 gene PCR product. Panel B reveals that Cx43 mRNA was only detected highly in the Cx43- transfected fibroblasts. In addition, low level expression of Cx43 was found in MyoD gene-transfected group. Non-treated fibroblasts did not express mRNA of these two genes; (C) MyoD and (D) Cx43 protein level was analyzed by western blotting. GAPDH was used as a sample loading control. MyoD or Cx43 protein was expressed in the MyoD- or Cx43-transfected fibroblasts. Note low level expression of Cx43 was found in MyoD gene-transfected fibroblasts.

    Techniques Used: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    Transfected-Myo D and Cx43 genes enable morphology conversion of fibroblast to myoblast like cells. Positive clones of four groups of fibroblasts: (A) non-treated fibroblast (N), (B) MyoD gene-transfected fibroblast (M), (C) Cx43 gene-transfected fibroblast (C) and (D) MyoD-Cx43-transfected fibroblast (MC) were screened out after lentivirus with Cx43 or MyoD gene transfection into rat fibroblast cell line RFL-6. The figures show that RFL-6 cells in M and MC group had changed with cell elongation, cytoplasm enrichment and granule manifold and become myoblast like cells. Cell morphology of C group was similar with N group but proliferation ability decreased obviously (data not shown).
    Figure Legend Snippet: Transfected-Myo D and Cx43 genes enable morphology conversion of fibroblast to myoblast like cells. Positive clones of four groups of fibroblasts: (A) non-treated fibroblast (N), (B) MyoD gene-transfected fibroblast (M), (C) Cx43 gene-transfected fibroblast (C) and (D) MyoD-Cx43-transfected fibroblast (MC) were screened out after lentivirus with Cx43 or MyoD gene transfection into rat fibroblast cell line RFL-6. The figures show that RFL-6 cells in M and MC group had changed with cell elongation, cytoplasm enrichment and granule manifold and become myoblast like cells. Cell morphology of C group was similar with N group but proliferation ability decreased obviously (data not shown).

    Techniques Used: Transfection, Clone Assay

    Expression of Desmin and α-actin in different transfected group were analyzed by western blotting. The antibodies of α-actin and Desmin were muscle cell specific. GAPDH was also detected as a sample loading control. The figure shows that MyoD downstream molecules, Desmin and α-actin, were all detected in cells transfected with MyoD or MyoD plus Cx43 genes but not in the cells transfected with Cx43 gene alone or control.
    Figure Legend Snippet: Expression of Desmin and α-actin in different transfected group were analyzed by western blotting. The antibodies of α-actin and Desmin were muscle cell specific. GAPDH was also detected as a sample loading control. The figure shows that MyoD downstream molecules, Desmin and α-actin, were all detected in cells transfected with MyoD or MyoD plus Cx43 genes but not in the cells transfected with Cx43 gene alone or control.

    Techniques Used: Expressing, Transfection, Western Blot, Control



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    Image Search Results


    Culture of packaging cells 293FT and production of lentivirus. (A) Morphology of 293FT cells before viral packaging. The packaging cells were shown in good condition; (B) Morphology of 293FT cells during ingathering. The pLenti-based expression vector containing gene of rat Cx43 or MyoD and the ViraPower packing were transfected into 293FT cell line to produce lentivirus. The viruses were ingathered after culturing for 72h.

    Journal: International Journal of Biomedical Science : IJBS

    Article Title: Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro

    doi:

    Figure Lengend Snippet: Culture of packaging cells 293FT and production of lentivirus. (A) Morphology of 293FT cells before viral packaging. The packaging cells were shown in good condition; (B) Morphology of 293FT cells during ingathering. The pLenti-based expression vector containing gene of rat Cx43 or MyoD and the ViraPower packing were transfected into 293FT cell line to produce lentivirus. The viruses were ingathered after culturing for 72h.

    Article Snippet: Rabbit primary polyclonal antibody against MyoD and Cx43 were obtained from Santa Cruz (USA), while rabbit anti-rat Desmin and α-actin were from Lab Vision.

    Techniques: Expressing, Plasmid Preparation, Transfection

    Expression of rat Cx43 and MyoD after lentivirus transfection. MyoD and Cx43 expression levels in four groups of fibroblasts: non-treated fibroblast (N), MyoD gene-transfected fibroblast (M), Cx43 gene-transfected fibroblast (C) and MyoD-Cx43-transfected fibroblast (MC) were analyzed. (A) MyoD mRNA expression was determined by RT-PCR. The 254bp band is MyoD gene amplifying product. The figure shows that MyoD mRNA was highly expressed in the MyoD- and MyoD plus Cx43- transfected fibroblasts; (B) Cx43 mRNA expression was determined by RT-PCR. The 580 bp band indicates Cx43 gene PCR product. Panel B reveals that Cx43 mRNA was only detected highly in the Cx43- transfected fibroblasts. In addition, low level expression of Cx43 was found in MyoD gene-transfected group. Non-treated fibroblasts did not express mRNA of these two genes; (C) MyoD and (D) Cx43 protein level was analyzed by western blotting. GAPDH was used as a sample loading control. MyoD or Cx43 protein was expressed in the MyoD- or Cx43-transfected fibroblasts. Note low level expression of Cx43 was found in MyoD gene-transfected fibroblasts.

    Journal: International Journal of Biomedical Science : IJBS

    Article Title: Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro

    doi:

    Figure Lengend Snippet: Expression of rat Cx43 and MyoD after lentivirus transfection. MyoD and Cx43 expression levels in four groups of fibroblasts: non-treated fibroblast (N), MyoD gene-transfected fibroblast (M), Cx43 gene-transfected fibroblast (C) and MyoD-Cx43-transfected fibroblast (MC) were analyzed. (A) MyoD mRNA expression was determined by RT-PCR. The 254bp band is MyoD gene amplifying product. The figure shows that MyoD mRNA was highly expressed in the MyoD- and MyoD plus Cx43- transfected fibroblasts; (B) Cx43 mRNA expression was determined by RT-PCR. The 580 bp band indicates Cx43 gene PCR product. Panel B reveals that Cx43 mRNA was only detected highly in the Cx43- transfected fibroblasts. In addition, low level expression of Cx43 was found in MyoD gene-transfected group. Non-treated fibroblasts did not express mRNA of these two genes; (C) MyoD and (D) Cx43 protein level was analyzed by western blotting. GAPDH was used as a sample loading control. MyoD or Cx43 protein was expressed in the MyoD- or Cx43-transfected fibroblasts. Note low level expression of Cx43 was found in MyoD gene-transfected fibroblasts.

    Article Snippet: Rabbit primary polyclonal antibody against MyoD and Cx43 were obtained from Santa Cruz (USA), while rabbit anti-rat Desmin and α-actin were from Lab Vision.

    Techniques: Expressing, Transfection, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control

    Transfected-Myo D and Cx43 genes enable morphology conversion of fibroblast to myoblast like cells. Positive clones of four groups of fibroblasts: (A) non-treated fibroblast (N), (B) MyoD gene-transfected fibroblast (M), (C) Cx43 gene-transfected fibroblast (C) and (D) MyoD-Cx43-transfected fibroblast (MC) were screened out after lentivirus with Cx43 or MyoD gene transfection into rat fibroblast cell line RFL-6. The figures show that RFL-6 cells in M and MC group had changed with cell elongation, cytoplasm enrichment and granule manifold and become myoblast like cells. Cell morphology of C group was similar with N group but proliferation ability decreased obviously (data not shown).

    Journal: International Journal of Biomedical Science : IJBS

    Article Title: Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro

    doi:

    Figure Lengend Snippet: Transfected-Myo D and Cx43 genes enable morphology conversion of fibroblast to myoblast like cells. Positive clones of four groups of fibroblasts: (A) non-treated fibroblast (N), (B) MyoD gene-transfected fibroblast (M), (C) Cx43 gene-transfected fibroblast (C) and (D) MyoD-Cx43-transfected fibroblast (MC) were screened out after lentivirus with Cx43 or MyoD gene transfection into rat fibroblast cell line RFL-6. The figures show that RFL-6 cells in M and MC group had changed with cell elongation, cytoplasm enrichment and granule manifold and become myoblast like cells. Cell morphology of C group was similar with N group but proliferation ability decreased obviously (data not shown).

    Article Snippet: Rabbit primary polyclonal antibody against MyoD and Cx43 were obtained from Santa Cruz (USA), while rabbit anti-rat Desmin and α-actin were from Lab Vision.

    Techniques: Transfection, Clone Assay

    Expression of Desmin and α-actin in different transfected group were analyzed by western blotting. The antibodies of α-actin and Desmin were muscle cell specific. GAPDH was also detected as a sample loading control. The figure shows that MyoD downstream molecules, Desmin and α-actin, were all detected in cells transfected with MyoD or MyoD plus Cx43 genes but not in the cells transfected with Cx43 gene alone or control.

    Journal: International Journal of Biomedical Science : IJBS

    Article Title: Experimental Studies on the Differentiation of Fibroblasts into Myoblasts induced by MyoD Genes in vitro

    doi:

    Figure Lengend Snippet: Expression of Desmin and α-actin in different transfected group were analyzed by western blotting. The antibodies of α-actin and Desmin were muscle cell specific. GAPDH was also detected as a sample loading control. The figure shows that MyoD downstream molecules, Desmin and α-actin, were all detected in cells transfected with MyoD or MyoD plus Cx43 genes but not in the cells transfected with Cx43 gene alone or control.

    Article Snippet: Rabbit primary polyclonal antibody against MyoD and Cx43 were obtained from Santa Cruz (USA), while rabbit anti-rat Desmin and α-actin were from Lab Vision.

    Techniques: Expressing, Transfection, Western Blot, Control